Microfluidics, Lab- & Organ-on-a-Chip – Infrastructure

INFRASTRUCTURE

The group operates a microfluidics fabrication and related characterization laboratory (in collaboration with IPRETEA), as well as infrastructure for microfluidics simulation. Our infrastructure comprises the following main equipment.

I. Microfluidics fabrication

Hydraulic heated press (Carver 3850) for hot embossing-based pattern formation on polymeric substrates and sealing of polymeric microfluidics

Ultrasonic bonding machine for sealing polymeric/plastic microfluidic devices (HiQ EVOLUTION 1200 by Herrmann Ultraschalltechnik GmbH & Co.)

Dry Film Roll Laminator (Model 305, Fortex) for sealing films, foils and dry photoresists. Sealing and fabrication of microfluidic chips (equipment borrowed from INRASTES, NCSR “Demokritos”)

II. Microfluidics characterization & operation

Various micropumps (syringe & peristaltic ones, such as SPS01-Lab Smith and P625-INSTECH) for flow characterization and operation of microfluidics

Thermal Imaging Camera FLIR A325sc (320 x 240 resolution) with standard 25° lens and close-up 4X lens (100 μm resolution) for real-time analysis of temperature of heated microdevices (e.g. microfluidic devices with integrated microheaters)

UV-NIR Spectrophotometer (from Theta-metrisis) for absorption measurements of liquid samples in microfluidics and lab-on-chip devices

Custom-made temperature controller units to drive microheaters integrated in microfluidics

Rapid Temperature Thermocycling system with a temperature controller and Peltier elements. The system includes a temperature sensor and a heat sink (from BELECTRONIG GMBH)

High Voltage Sequencer (from LabSmith, HVS448) for microfluidics control (capillary electrophoresis, electrowetting, etc.)

 

III. Equipment for DNA amplification experiments

The group performs DNA amplification experiments for microfluidics validation in collaboration with INRASTES, NCSR “Demokritos”. Most of the relevant equipment is owned by and located at INRSTES. Specifically, a iCycler™ thermal cycler (from BIO-RAD), a UV transilluminator (MiniBIS Pro) for agarose gel visualization and a gel electrophoresis system (owned by the group) for nucleic acid separations.

Gel Electrophoresis System for nucleic acid separations. It includes a gel tray and a power supply (BIORAD, WIDE Mini-Sub Cell GT Electrophoresis SYSTEM 15X10 cm TRAY & POWER-PAC BASIC POWER SUPPLY)

IV. Microfluidics simulation Facility

The Simulation Facility includes a computer cluster running commercial & open-source software (COMSOL Multiphysics & ANSYS for microfluidics simulations).