Microfluidics, Lab- & Organ-on-a-Chip – Publications-Journals

2020 

Fabrication of a 3D microfluidic cell culture device for bone marrow-on-a-chip
D. Kefallinou, M. Grigoriou, D.T. Boumpas, E. Gogolides and A. Tserepi
Micro & Nano Engineering 100075

Lab-on-Chip platform and protocol for rapid foodborne pathogen detection comprising on-chip cell capture, lysis, DNA amplification and surface-acoustic-wave detection
K. Tsougeni, G.D. Kaprou, C-M. Loukas, G. Papadakis, A. Hamiot, M. Eck, D. Rabus, G. Kokkoris, S. Chatzandroulis, V. Papadopoulos, B. Dupuy, G. Jobst, E. Gizeli, A. Tserepi, E. Gogolides
Sensors and Actuators B: Chem. 128345

Poly-L-histidine coated microfluidic devices for bacterial DNA purification without chaotropic solutions
A. S. Kastania, P. S. Petrou, C-M. Loukas, E. Gogolides
Biomedical Microdevices 22(3) 1-12

Towards PCB-Based Miniaturized Thermocyclers for DNA Amplification
G.D. Kaprou, V. Papadopoulos, C-M. Loukas, G. Kokkoris, A. Tserepi
Micromachines 11 (3), 258

2019

Detection of BRCA1 gene on partially reduced graphene oxide biosensors
M.K. Filippidou, C-M. Loukas, G.D. Kaprou, PS. Petrou, S. Kakambakos, A. Tserepi, S. Chatzandroulis
Microel. Engineering 216, 111093

Ultrafast, low-power, PCB manufacturable, continuous-flow microdevice for DNA amplification
G.D. Kaprou, V. Papadopoulos, D.P. Papageorgiou, I. Kefala, G. Papadakis, E. Gizeli, S. Chatzandroulis, G. Kokkoris, A. Tserepi
Anal. Bioanal. Chem. 1-11

A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening
K. Tsougeni, A. Kastania, G.D. Kaprou, M. Eck, G. Jobst, P.S. Petrou, S.E. Kakambakos, D. Mastellos, E. Gogolides, A. Tserepi
Sensors and Actuators B: Chem. 288, pp 171-179

Gradient-temperature hot-embossing for dense micropillar array fabrication on thick cyclo-olefin polymeric plates: An example of a microfluidic chromatography column fabrication
I. Kourmpetis, A.S. Kastania, K. Ellinas, K. Tsougeni, M. Baca, W. De Malsche, E. Gogolides
Micro and Nano Engineering 5, art. no. 100042

Plug actuation and active manipulation in closed monolithic fluidics using backpressure
N. Vourdas, G. Pashos, E. Rizos, G. Kokkoris, E. Tsampasis, E. Klouvidaki, A.G. Boudouvis, V.N. Stathopoulos
Microelectronic Engineering 216, art. no. 111046

2018

Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays: Comparison with epoxy-silane coated glass slides
K. Tsougeni, K. Ellinas, G. Koukouvinos, P.S. Petrou, A. Tserepi, S. E. Kakabakos, E. Gogolides
Colloids & Surfaces B: Biointerfaces 165, pp 270-277

Stable hydrophilization of FR4 and polyimide-based substrates implemented in microfluidics-on-PCB
E. Cunaj, P.S. Petrou, G.D. Kaprou, S.E. Kakabakos, E. Gogolides, A. Tserepi
Surf. & Coat. Technology 334, pp 292-299

3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning
K. Tsougeni, K. Ellinas, G. Koukouvinos, P.S. Petrou, A. Tserepi, S. E. Kakabakos, E. Gogolides
Cell-based Microarrays Springer 165, pp 27-40

2017

Micro-bead immunoassays for the detection of IL6 and PDGF-2 proteins on a microfluidic platform, incorporating superhydrophobic passive valves
K. Ellinas, V. Pliaka, G. Kanakaris A. Tserepi, L.G. Alexopoulos, E. Gogolides
Microelectronic Engineering 175, pp 73–80

Βinding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments
A.S. Kastania, K. Tsougeni, V. Constantoudis, E. Gogolides
Sensors and Actuators, B: Chemical 253, 247-257

Polymeric Microfluidics: Fabricated and Modified Using Plasmas
K. Tsougeni, A. Tserepi, E. Gogolides
Encyclopedia of Plasma Technology DOI: 10.1081/E-EPLT-120053912, Copyright © 2017 by Taylor & Francis, p. 1112-1124

The Lab-on-PCB approach: Tackling the mTAS commercial upscaling bottleneck (Critical Review)
D. Moschou, A. Tserepi
Lab-Chip 17, pp 1388-1405

2016

Plasma micro-nanotextured polymeric micromixer for DNA purification with high efficiency and dynamic range
A.S. Kastania, K. Tsougeni, G. Papadakis, E. Gizeli, G. Kokkoris, A. Tserepi, E. Gogolides
Analytica Chimica Acta 26, pp 58-67

Nanostructured PMMA-coated Love wave device as a platform for protein adsorption
M. Gianneli, K. Tsougeni, A. Tserepi, E. Gogolides, E. Gizeli
Sensors and Actuators B: Chem. 236, pp 583-590

Miniaturized devices for isothermal DNA amplification addressing DNA diagnostics
G. D. Kaprou, G. Papadakis, D. P. Papageorgiou, G. Kokkoris, V. Papadopoulos, I. Kefala, E. Gizeli, A. Tserepi
Microsyst. Technol. 22(7), pp 1529–1534

Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis
K. Tsougeni, G. Papadakis, M. Gianneli, A. Grammoustianou, V. Constantoudis, B. Dupuy, P. N. Petrou, S. E. Kakabakos, A. Tserepi, E. Gizeli, E. Gogolides
Lab-Chip 16, pp 120-131

Micro-nano-bio diagnostic system for food pathogen detection revolutionizes food safety management and protects consumers health
E. Gogolides, A. Tserepi, G. Jobst, J-M. Friedt, D. Rabus, Z. Bilkova, S. Decroix, J-L. Viovy, G. Papadakis, E. Gizeli
Studies in Health Technology and Informatics 224, pp. 67-72

Droplet Mobility Manipulation on porous media using backpressure
N. Vourdas, G. Pashos, G. Kokkoris, A.G. Boudouvis, V.N. Stathopoulos
Langmuir 32(21), pp 5250-5258

2015

Comparison of continuous‑flow and static‑chamber μPCR devices through a computational study: the potential of flexible polymeric substrates
V. E. Papadopoulos, G. Kokkoris, I. N. Kefala, A. Tserepi
Microfluid Nanofluid 19, pp 867-882

A labyrinth split and merge micromixer for bioanalytical applications
I. N. Kefala, V. E. Papadopoulos, G. Kaprou, G. Kokkoris, G. Papadakis, A. Tserepi
Microfluid Nanofluid 19, pp 1047-1059

A microfabricated cyclo-olefin polymer microcolumn used for reversed-phase chromatography
K. Tsougeni, K. Ellinas, H. Archontaki, E. Gogolides
Journal of Micromechanics and Microengineering 25 (1), art. no. 015005

Hierarchical micro and nano structured, hydrophilic, superhydrophobic and superoleophobic surfaces incorporated in microfluidics, microarrays and lab on chip microsystems (review)
E. Gogolides, K. Ellinas, A. Tserepi
Microelectronic Engineering 132, 135-155

Miniaturized devices towards an Integrated Lab-on-a-chip Platform for DNA diagnostics
G. Kaprou, G. Papadakis, G. Kokkoris, V. Papadopoulos, I. Kefala, D. Papageorgiou, E. Gizeli, A. Tserepi
Bio-MEMS and Medical Microdevices II, edited by Sander van den Driesche, Proc. of SPIE Vol. 9518, 95180G-1

2014

Photolithography and plasma processing of polymeric lab on chip for wetting and fouling control and cell patterning
K. Tsougeni, A. Bourkoula, P. Petrou, A. Tserepi, S.E. Kakabakos, E. Gogolides
Microelectronic Engineering 124, 47-52

A passive micromixer for enzymatic digestion of DNA
V.E. Papadopoulos, I.N. Kefala, G. Kaprou, G. Kokkoris, D. Moschou, G. Papadakis, E. Gizeli, A. Tserepi
Microelectronic Engineering 124, 42-46

All-plastic, low-power, disposable, continuous-flow PCR chip with integrated microheaters for rapid DNA amplification
D. Moschou, N. Vourdas, G. Kokkoris, G. Papadakis, J. Parthenios, S. Chatzandroulis, A. Tserepi
Sensors & Actuators B: Chem. 199, pp 470-478

Superhydrophobic, passive microvalves with controllable opening threshold: exploiting plasma nanotextured microfluidics for a programmable flow switchboard
K. Ellinas, A. Tserepi, E. Gogolides
Microfluidics & Nanofluidics 17, pp 489

2013

Plasma-Assisted Nanoscale Protein Patterning on Si Substrates via Colloidal Lithography
A. Malainou, K. Tsougeni, K. Ellinas, P. S. Petrou, V. Constantoudis, E. Sarantopoulou, K. Awsiuk, A. Bernasik, A. Budkowski, A. Markou, I. Panagiotopoulos, S. E. Kakabakos, E. Gogolides, A. Tserepi
Journal of Physical Chemistry A 117, pp 13743-13751

Phosphopeptide enrichment and separation in an affinity microcolumn on a silicon microchip: Comparison of sputtered and wet-deposited TiO2 stationary-phase
K. Ellinas, K. Tsougeni, G. Boulousis, Th. Speliotis, A. Tserepi, E. Gogolides
Sensors & Actuators B 188, pp 1073-1079

Reversible pressure-induced switching of droplet mobility after impingement on porous surface media
N. Vourdas, A. Tserepi, V. N. Stathopoulos
Appl. Phys. Lett. 103, pp 111602

Plasma etching of poly(dimethylsiloxane): roughness formation, mechanism, control, and application in the fabrication of microfluidic structures
M-E. Vlachopoulou, G. Kokkoris, C. Cardinaud, E. Gogolides, A. Tserepi
Plasma Processes and Polymers 10, pp 29-40

Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors
D. Moschou, N. Vourdas, M.K. Filippidou , V. Tsouti , G. Kokkoris, G. Tsekenis, I. Zergioti, S. Chatzandroulis, A. Tserepi
SPIE Proceedings Vol. 8765, Bio-MEMS and Medical Microdevices; 87650L

2012

High Capacity and High Intensity DNA Microarray Spots Using Oxygen-Plasma Nanotextured Polystyrene Slides
K. Tsougeni, P. S. Petrou, A. Tserepi, S. E. Kakabakos, E. Gogolides
Anal. Bioanal. Chem. 403, pp 2757-2764

Creating highly dense and uniform protein and DNA microarrays through Photolithography and Plasma Modification of Glass Substrates
A. Malainou, P. S. Petrou, S. E. Kakabakos, E. Gogolides, A. Tserepi
Biosensors & Bioelectronics 34(1), pp 273-281

Superior performance of multilayered fluoropolymer films in low voltage electrowetting
D. Papageorgiou, A. Tserepi, A. Boudouvis, A. Papathanasiou
J. Colloid Interface Science 368, pp 592-598

Flame aerosol deposition of TiO2 nanoparticle films on polymers and polymeric microfluidic devices for on-chip phosphopeptide enrichment
T. Rudin, K. Tsougeni, E. Gogolides, S.E. Pratsinis
Microelectronic Engineering 97, pp. 341-344

Controlled protein adsorption on microfluidic channels with engineered roughness and wettability
K.Tsougeni, P. Petrou, D. Papageorgiou, S. Kakabakos, A. Tserepi, E. Gogolides
Sensors & Actuators: B. Chemical 161(1), pp 216-222

Fabrication of a label-free micromechanical capacitive biosensor and integration with μPCR towards a LoC for disease diagnosis
D. Moschou, N. Vourdas, G. Kokkoris, G. Tsekenis, V. Tsouti, I. Zergioti, A. Tserepi, S. Chatzandroulis
Proc. of the 16th Int. Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS)

Development of a continuous-flow μPCR device with microheating elements integrated with biosensors towards a LoC system for disease diagnosis
N. Vourdas, D. Moschou, G. Kokkoris, G. Papadakis, S. Chatzandroulis, A. Tserepi
Proc. of 3rd European Microfluidics Conference, paper no. 128

Flow study in randomly-rough superhydrophilic and superhydrophobic plasma nanotextured microchannels using micro-PIV
K. Tsougeni, K. Ellinas, A. Glynou, T. Christoforidis, D.S. Mathioulakis, A. Tserepi, E. Gogolides
Proc. of 3rd European Microfluidics Conference, paper no. 132

Control of flow and protein adsorption on plasma nanotextured microfluidics
K. Ellinas, K. Tsougeni, P.S. Petrou, D.P. Papageorgiou, S.E. Kakabakos, A. Tserepi, E. Gogolides
Proc. of 3rd European Microfluidics Conference, paper no. 133

2011

TiO2-ZrO2 Affinity Chromatography Polymeric Microchip for Phosphopeptide Enrichment and Separation
K. Tsougeni, P. Zerefos, A. Tserepi, A. Vlachou, S. Garbis, E. Gogolides
Lab Chip 11(18), pp 3113-3120

Protein arrays on high-surface area plasma-nanotextured poly(dimethylsiloxane)-coated glass slides
M-E. Vlachopoulou, A. Tserepi, P.S. Petrou, E. Gogolides, S.E. Kakambakos
Colloids and Surfaces B: Biointerfaces 83, pp 270-276

Fabrication and modeling of a continuous-flow microfluidic device for on-chip DNA amplification
G. Kokkoris, D. C. Moschou, E. Mavraki, S. Chatzandroulis, A. Tserepi
Proc. of 3rd Micro and Nanoflows Conference, Brunel Institute for Bioengineering (BIB)

A continuous flow µPCR device with integrated microheaters on a flexible polyimide substrate
E. Mavraki, D. Moschou, G. Kokkoris, N. Vourdas, S. Chatzandroulis, A. Tserepi
Proc. of Eurosensors XXV, Procedia Engineering 25, pp. 1245-1248

Nanoscale protein patterning on Si substrates using colloidal lithography and plasma processing
A. Malainou, K. Ellinas, P.S. Petrou, S.E. Kakabakos, V. Constantoudis, E. Gogolides, A. Tserepi
Proc. of Eurosensors XXV, Procedia Engineering 25, pp. 1641-1644

Plasma nanotextured Polystyrene for intense DNA microarrays
K. Tsougeni, P.S. Petrou, A. Tserepi, S.E. Kakabakos, E. Gogolides
Proc. of Eurosensors XXV, Procedia Engineering 25, pp. 1573-1576

TiO2 Affinity Chromatography Microcolumn on Si Substrates for Phosphopeptide Analysis
G. Boulousis, K. Tsougeni, K. Ellinas, A. Speliotis, A. Tserepi, E. Gogolides
Proc. of Eurosensors XXV, Procedia Engineering 25, pp. 717-720

2010

Plasma Nanotextured PMMA surfaces for protein arrays: Increased protein binding and enhanced detection sensitivity
K. Tsougeni, A. Tserepi, V. Constantoudis, E. Gogolides, P.S. Petrou, S.E. Kakabakos
Langmuir 26(17), pp 13883-13891

Smart multifunctional Polymeric Microfluidics Fabricated by Plasma Processing: Applications in Capillary Filling, and Passive Superhydrophobic Valving
K. Tsougeni, D. Papageorgiou, A. Tserepi, E. Gogolides
Lab Chip 10, pp 462-469

Protein arrays on high-surface-area plasma-nanotextured poly(dimethylsiloxane)-coated glass slides
M-E. Vlachopoulou, A. Tserepi, P.S. Petrou, E. Gogolides, S.E. Kakabakos
Colloids and Surfaces B: Biointerfaces 83, pp 270-276

Study of flow and pressure field in microchannels with various cross-section areas
A. Petropoulos, G. Kaltsas, D. Randjelovic, E. Gogolides
Microelectronic Engineering 87 (5-8), pp. 827-829

2009

High density protein patterning through selective plasma-induced fluorocarbon deposition on Si substrates
P. Bayiati, A. Malainou, E. Matrozos, A. Tserepi, P.S. Petrou, S.E. Kakambakos, E. Gogolides
Biosensors & Bioelectronics 24, pp 2979-2984

SAW device integrated with microfluidics for array-type biosensing
Κ. Mitsakakis, A. Tserepi, E. Gizeli
Microelectronic Engineering 86, pp 1416-1418

A low temperature surface modification assisted method for bonding plastic substrates
M-E. Vlachopoulou, A. Tserepi, P. Pavli, P. Argitis. M. Sanopoulou, K. Misiakos
J. Micromech. Microeng. 19 015007 (6pp)

Evaluation of a microfluidic sensor fabricated on polymeric material
A. Petropoulos, K. Kontakis, G. Kaltsas, E. Gogolides
DTIP of MEMS and MOEMS – Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, art. no. 4919510, pp. 398-401

A flexible capacitive device for pressure and tactile sensing
A. Petropoulos, G. Kaltsas, D. Goustouridis, E. Gogolides
Procedia Chemistry 1 (1), pp. 867-870

A novel microfluidic integration technology for PCB-based devices: Application to microflow sensing
K. Kontakis, A. Petropoulos, G. Kaltsas, T. Speliotis, E. Gogolides
Microelectronic Engineering 86 (4-6), pp. 1382-1384

2008

High aspect-ratio plasma-induced nanotextured polydimethylsiloxane surfaces with enhanced protein adsorption capacity
M-E. Vlachopoulou, P.S. Petrou, S.E. Kakambakos, A. Tserepi, E. Gogolides
J. Vac. Sci. Technol. 26(6), pp 2543-2548

Fabrication of a micro-column for gas separation using poly(dimethylsiloxane) as structural and functional material
A. Malainou, M-E. Vlachopoulou, R. Triantafyllopoulou, A. Tserepi, S. Chatzandroulis
J. Micromech. Microeng. 18, 105007

Integration of Microfluidics with a Love Wave Sensor for the Fabrication of a Multi-Sample Analytical Microdevice
Κ. Mitsakakis, A. Tserepi, E. Gizeli
J. MicroElectroMechanical Systems 17 (4), pp 1010-1019

Plasma processing for polymeric microfluidics fabrication and surface modification: effect of superhydrophobic walls on electroosmotic flow
N. Vourdas, A. Tserepi, A.G. Boudouvis, E. Gogolides
Microelectronic Engineering 85, pp 1124-27

An Integrated Microfluidics-on-SAW (“μF-on-SAW”) Setup for Multi-Sample Sensing
K. Mitsakakis, A. Tserepi, E. Gizeli
Frequency Control Symposium, IEEE International, 337-340

2007

Novel microfluidic flow sensor based on a microchannel capped by porous silicon
D.N. Pagonis, A. Petropoulos, G. Kaltsas, A.G. Nassiopoulou, A. Tserepi
Physica Status Solidi (A) Applications and Materials 204 (5), pp 1474-1479

Electrowetting on plasma deposited fluorocarbon hydrophobic films for bio-fluid transport in microfluidics
P. Bayiati, A. Tserepi, P. S. Petrou, K. Misiakos, S. E. Kakabakos, E. Gogolides
J. Applied Physics 101, p 103306

Photosensitive Poly-dimethylsiloxane (PDMS) materials for Microfluidic Applications
K. Tsougeni, A. Tserepi, E. Gogolides
Microelectronic Engineering 84, pp 1104-1108

Biofluid transport on hydrophobic plasma deposited fluorocarbon films
P. Bayiati, A. Tserepi, P. S. Petrou, K. Misiakos, S. E. Kakabakos, E. Gogolides
Microelectronic Engineering 84, pp 1677-1680

Protein patterning through selective fluorocarbon plasma-induced deposition on silicon
P. Bayiati, A. Tserepi, P. S. Petrou, S. E. Kakabakos, E. Matrozos, E. Gogolides
Proc. of 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp. 92-94

2006

Monolithic silicon optoelectronic transducers and elastomeric fluidic modules for bio-spotting and bio-assay experiments
K. Misiakos, P.S. Petrou, S.E. Kakabakos, M.E. Vlahopoulou, A. Tserepi, E. Gogolides, H.H. Ruf
Microelectronic Engineering 83, pp 1605-1608

Monolithic silicon optoelectronic devices for protein and DNA detection
K. Misiakos, P. Petrou, S. E. Kakabakos, M-E. Vlachopoulou, A. Tserepi, E. Gogolides
Proceedings of SPIE – The International Society for Optical Engineering, art. no. 61250W

2005

Patterning of thick polymeric substrates for the fabrication of microfluidic devices
M-E. Vlachopoulou, A. Tserepi, N. Vourdas, E. Gogolides, K. Misiakos
Journal of Physics: Conference Series 10 (1), pp 293-296

2004

Selective Plasma-induced Deposition of Fluorocarbon Films on Metal Surfaces for actuation in microfluidics
P. Bayiati, A. Tserepi, E. Gogolides, K. Misiakos
J. Vac. Sci. Technnol. A 22(4), pp 1546-1551