Research Interests : Macromolecular crystallogenesis, X-ray crystallography of proteins (incl. complexes for structure-based drug design) and small organic molecules

Email : e.saridakis@inn.demokritos.gr

Research Interests
Macromolecular crystallogenesis, X-ray crystallography of proteins (incl. complexes for structure-based drug design) and small organic molecules
Position
Researchers
Telephone
+30 210 650 3793
CV English
About/Biography
Emmanuel Saridakis was born in Brussels in 1970. He obtained his Bachelor’s degree in Physics in 1991 and his Ph.D. in Biophysics/ Crystallography in 1995 (under the supervision of Professor David Blow, FRS), both from the Physics Department, Imperial College London. He also holds a Master’s degree in the Philosophy and History of Science from the London School of Economics and Political Science (2000).
He has worked as a post-doctoral Research Associate at the Biology Department, University of Athens, at the Physics Department and the Biomedical Sciences Division of Imperial College and at the Institute of Molecular Biology and Biotechnology/ FoRTH in Crete, Greece. He has also lectured at the Higher Technological Education Institute of Athens.
He is since 2018 a Principal Researcher at the Laboratory of Structural and Supramolecular Chemistry (elected Researcher D in 2008), Institute of Nanoscience & Nanotechnology, National Centre for Scientific Research “Demokritos” in Athens, and (since 2007) an Honorary Research Fellow at the Faculty of Medicine, Imperial College London.
He has served as a Council member of the International Organisation for Biological Crystallisation (2008-2014) and is presently a member of the Governing Council of the Hellenic Crystallographic Association. He has also served as referee for more than 16 scientific journals and various governmental funding agencies internationally.
His research interests focus mainly on crystallisation theory and the development of new methodology for macromolecular crystallisation. He has worked on the establishment and use of crystallisation phase diagrams for crystal optimisation, novel nucleation-inducing substrates and nucleation theory, crystallisation in gels, screening methodology, and on the miniaturisation and automation of the above. He is also very active in macromolecular and small organic molecule crystallography, including the structure elucidation of members of the crustacyanin and ferredoxin families, endoplasmic reticulum aminopeptidases (ERAP2, IRAP) and their complexes with inhibitors for structure-based drug design, and of Disulfide bond (Dsb) proteins.
He has published 42 full papers in peer-reviewed international journals (>2130 citations, h-factor 23), one textbook, and has given many national and international Conference talks, invited talks and posters, as well as lectures and seminars at Workshops and Summer Schools.

Selected publications:

1. Saridakis, E.E.G., Shaw Stewart, P.D., Lloyd, L.F., Blow, D.M. Phase diagram and dilution experiments in the crystallization of carboxypeptidase G2. Acta Crystallogr. D50, 293-297 (1994).

2. Chayen, N.E., Saridakis, E., El-Bahar, R., Nemirovsky, Y. Porous Silicon: an Effective Nucleation-inducing Material for Protein Crystallization. J. Mol. Biol. 312, 591-595 (2001).

3. Sears, R., Saridakis, E., Chayen, N.E. Experiment and theory for heterogeneous nucleation of protein crystals in a porous medium. Proc. Natl. Acad. Sci. U.S.A. 103, 597-601 (2006).

4. Chayen, N.E., Saridakis, E. Protein crystallization: from purified protein to diffraction-quality crystal. Nature Methods 5, 147-153 (2008).

5. Saridakis, E., Chayen, N.E. Towards a ‘universal’ nucleant for protein crystallization. Trends Biotechnol. 27, 99-106 (2009).

6. Saridakis, E. et al. Protein crystallization facilitated by Molecularly Imprinted Polymers. Proc. Natl. Acad. Sci. USA 108, 11081-11086 (2011).

7. Birtley, J., Saridakis, E., Stratikos E., Mavridis I.M. Crystal structure of human ER Aminopeptidase 2 reveals atomic basis for distinct roles in antigen processing. Biochemistry 51, 286-295 (2012).

8. Zervoudi, E., Saridakis, E., Birtley, J.R., Seregin, S., Reeves, E., Kokkala, P., Aldhamen, Y.A., Amalfitano, A., Mavridis, I.M., James, E., Georgiadis D., Stratikos, E. A rationally designed inhibitor targeting antigen-trimming aminopeptidases enhances antigen presentation and cytotoxic T-cell responses. Proc. Natl. Acad. Sci. USA 110, 19890-19895 (2013).

9. Khurshid, S., Saridakis, E., Govada, L., Chayen, N.E. Porous nucleating agents for protein crystallization. Nature Protocols 9, 1621-1633 (2014).

10. Mpakali, A., Giastas, P., Deprez-Poulain, R., Papakyriakou, A., Koumantou, D., Gealageas, R., Tsoukalidou, S., Vourloumis, D., Mavridis, I.M., Stratikos, E., Saridakis, E. Crystal structures of ER Aminopeptidase 2 in complex with inhibitors reveal pharmacophore requirements for optimizing inhibitor potency. ACS Med. Chem. Lett. 8, 333–337 (2017).