Smart Cycler II (Cepheid, Sunnyvale, CA, USA) is used with the Phytoxigene™ CyanoDTec (Diagnostic Technology, Belrose, Australia) multiplex quantitative real-time PCR reagents kit to determine the gene copies of the 16s rRNA gene (of total cyanobacteria) and the mcyE, cyrA, sxtA genes (of cyanotoxins microcystins, cylindrospermopsin, saxitoxins, respectively).