Cyanobacteria possess unique biosynthetic capabilities and produce a wide variety of cyanometabolites (CMs) with a broad spectrum of bioactivities. Arthrospira spp., commercially known as ‘Spirulina’, are gaining increasing interest in the food supplements sector due to their high content of proteins, nutrients, and bioactive compounds with beneficial properties. The isolation of these compounds faces a range of challenges and depends on the extraction techniques and conditions employed. The main objectives of this activity are (a) the development of appropriate protocols for the extraction/fractionation and characterization of cyanometabolites from cyanobacteria biomass using advanced analytical methods and tools (LC-MS/MS, HRM),

and (b) the investigation of their rich potential by assessing the biological activity of new bioactive compounds employing a battery of in vitro bioactivity assays. The results of this activity are expected to have a significant impact on the economy by attracting interest from various market sectors, such as natural pharmaceuticals, cosmetic ingredients and functional foods, driven by increasing consumer demand for high-added-value “green” products with unique properties.