Air Liquid Interface Exposure system
Tool
Essentials, Vitrocell
Applications
- Inhalation toxicology / respiratory toxicology
- Identification of biomarkers
- Drug delivery / inhaled therapeutics
- Nanomaterial / nanoparticle toxicity & safety
- Air pollution / environmental exposure studies
- Smoking / e-cigarette / vaping product testing
- Comparative exposure modes / methodology development
- Dosimetry / deposition quantification studies
- Extended / long-term exposuresHigh-throughput / multi-well exposures
- Organotypic / 3D respiratory tissue models
- Mechanistic studies & molecular endpoints
Technical Specifications
| Parameter | Specification |
| Exposure principle | Air–liquid interface exposure; cells cultured on porous membranes with apical air exposure |
| Exposure type | Aerosols, gases, nanoparticles, or vaporized compounds |
| Exposure mode | Continuous flow, single droplet (Vitrocell Cloud), or dry powder deposition (Vitrocell PowderX) |
| Number of exposure wells | Typically 6 or 12 exposure positions per module (expandable) |
| Exposure uniformity | ±10 % across wells using calibrated flow and aerosol distribution systems |
| Gas flow control | Mass flow controllers with individual channel regulation |
| Humidity & temperature control | Maintained at 85–95 % RH, 37 °C for cell viability and reproducibility |
| CO₂ concentration | Typically 5 % adjustable via integrated gas mixing module |
| Deposition monitoring | Quartz Crystal Microbalance (QCM) or tracer-based dosimetry for real-time dose measurement |
| Exposure duration | Short-term (minutes) to long-term (up to 72 h continuous exposure) |
| Compatible cell culture inserts | Standard 6-, 12-, or 24-well Transwell® or ThinCert® inserts |
| Materials of construction | High-grade stainless steel and borosilicate glass; autoclavable components |
| Automation | Optional integration with Vitrocell Automated Exposure Station for sequential or extended studies |
| Data acquisition | Real-time measurement of flow, temperature, humidity, COâ‚‚, and deposited dose |
Additional Tools
- VOC generator system
- Conditioning chamber
- Multi gas analyser Thermal desorption unit
Images
